Kirstine Callø

Associate professor, Ph.D.
kirstinec@sund.ku.dk  

Research Profile:

The main focus of the research in the Cellular Electrophysiology Laboratory is on electrophysiology and ion channels. In particular, we are interested in how mutations or pharmacological manipulations of ion channels affect the cardiac action potential and how the contraction of the heart is coordinated. We have extensive experience with heterologously expressed ion channels (mammalian cell lines and Xenopus laevis oocytes) as well as with recordings of native ionic currents in isolated cardiomyocytes. Further, we have established methods to record action potentials from multicellular preparations of equine hearts (the wedge model) as well as from various types of stem cells. 

Publications

53) Wang K, Preisler SS, Zhang L, Cui Y, Missel JW, Grønberg C, Gotfryd K, Lindahl E, Andersson M, Calloe K, Egea PF, Klaerke DA, Pusch M, Pedersen PA, Zhou ZH, Gourdon P. Structure of the human ClC-1 chloride channel. Accepted in PLOS Biology
IF: 9.163 

52) Pedersen SH, la Cour SH, Calloe K, Hauser F, Olesen J, Klaerke DA, Jansen-Olesen I. PACAP-38 and PACAP(6-38) degranulate rat meningeal mast cells via the orphan MrgB3-receptor. Accepted in Frontiers in Cellular Neuroscience
IF: 4.555 

*51) Grubb SJ, Vestergaard ML, Andersen AS, Rasmussen KK, Mamsen L, Tuckute G, Grunnet-Lauridsen K, Møllgård K, Ernst E, Christensen ST*, Calloe K*, Andersen CY.* Comparison of cultured human cardiomyocyte clusters obtained from embryos/fetuses or derived from human embryonic stem cells. Stem Cells Dev. 2019 Feb 13 *Eq. contributing.
IF: 3.315 

50) Zhang L, Wang K, Klaerke DA, Calloe K, Lowrey L, Pedersen PA, Gourdon P, Gotfryd K. Purification of Functional Human TRP Channels Recombinantly Produced in Yeast. Cells. 2019 Feb 11;8(2)
IF: 4.829 

49) Calloe K, Rognant S, Friis S, Shaughnessy C, Klaerke DA, Trachsel D. Compounds commonly used in equine medicine inhibits the voltage-gated potassium channel K_v11.1. Res Vet Sci. 2019 Jan 8;123:239-246
IF: 1.616 

*48) Calloe K. Doctoral Dissertation: The transient outward potassium current in healthy and diseased hearts. Acta Physiol (Oxf). 2019, 225 Suppl 717:e13225.
IF: 5.93 

47) Calloe K, Aistrup GL, Di Diego JM, Goodrow RJ, Treat JA, Cordeiro JM. Interventricular differences in sodium current and its potential role in Brugada syndrome. Physiol Rep. 2018 Jul;6(14):e13787
IF: 1.61 

46) Trachsel DS, Tejada MA, Christensen VG, Pedersen PJ, Kanters JK, Buhl R, Calloe K, Klaerke DA. Effects of Trimethoprim-Sulfadiazine and Detomidine on the function of equine Kv11.1 channels in a two-electrode voltage-clamp (TEVC) oocyte model. J Vet Pharmacol Ther. 2018 Aug;41(4):536-545
IF: 1.202

45) Klaerke DA, Tejada MA, Christensen VG, Lassen M, Pedersen PA, Calloe K. Reconstitution and Electrophysiological Characterization of Ion Channels in Lipid Bilayers. Current Protocols in Pharmacology 2018, 80:11.22.1–11.22.17.
IF: 1.28 

*44) Calloe K*, Broendberg AK*, Christensen AH, Pedersen LN, Olesen MS, Tejada MA, Friis S, Thomsen MB, Bundgaard H, Jensen HK. Multifocal atrial and ventricular premature contractions with an increased risk of dilated cardiomyopathy caused by a G213D Nav1.5 gain-of-function mutation. * Contributed equally to this work. J Int Card 2018 Apr 15;257:160-167
IF: 6.189 

*43) Vestergaard ML, Grubb SJ, Rasmussen KK, Anderson-Jenkins Z, Grunnet-Lauridsen K, Calloe K, Clausen C, Christensen ST, Møllgård K, Andersen CY. Human Embryonic Stem Cell derived Cardiomyocytes self-arrange with areas of different subtypes during differentiation. Stem Cells Dev. 2017 Nov 1;26(21):1566-1577
IF: 3.562 

42) Tejada MA, Hashem N, Calloe K, Klaerke DA. Heteromeric Slick/Slack K+ channels show graded sensitivity to cell volume changes. PLoS One. 2017 Feb 21;12(2):e0169914
IF: 4.411 

41) Zhang Y, Schmid B, Nikolaisen NK, Rasmussen MA, Aldana BI, Agger M, Calloe K, Sturmann TC, Larsen HM, Nielsen TT, Huang J, Xu F, Liu X, Bolund L, Meyer M, Bak LK, Waage-Petersen HS, Luo Y, Nielsen JE, The FReJA Consortium, Holst B, Clausen C, Hyttel P, Freude KK. Patient iPSC-Derived Neurons for Disease Modeling of Frontotemporal Dementia with Mutation in CHMP2B. Stem Cell Reports. 2017 Mar 14;8(3):648-658
IF: 7.023 

40) Speerschneider T, Grubb S, Olesen SP, Calloe K, Thomsen MB. Ventricular repolarization time, location of pacing stimulus and current pulse amplitude conspire to determine arrhythmogenicity in mice. Acta Physiol (Oxf). 2017 Mar;219(3):660-668
IF: 4.066 

*39) Calloe K, Di Diego JM, Hansen RS, Nagle S, Treat JA, Cordeiro JM. A Dual Potassium Channel Activator Improves Repolarization Reserve and Normalizes Ventricular Action Potentials. Biochem Pharmacol. 2016 Mar 18. pii: S0006-2952(16)00182-9.
IF: 5.091  

38) Thomsen MB, Calloe K. Human atrial fibroblasts and their contribution to supraventricular arrhythmia. Physiol Rep. 2016 Feb;4(3). pii: e12711. Editorial on Poulet et al., Altered physiological functions and ion currents in atrial fibroblasts from patients with chronic atrial fibrillation. Physiol Rep. 2016 Feb;4(2). pii: e12681.
IF: 1.61 

37) Cordeiro JM*, Calloe K*, Aschar-Sobbi R*, Kim K-H, Korogyi A, Occhipinti D, Backx PH, Panama BK. Physiological roles of the transient outward current Ito in normal and diseased hearts. Front Biosci (Schol Ed). 2016 Jan 1;8:143-59. * Contributed equally to this work. Review
IF: 2.60 

36) Pedersen PJ, Thomsen KB, Olander ER, Hauser F, Tejada MA, Poulsen KL, Grubb S, Buhl R, Calloe K, Klaerke DA. Molecular cloning and functional expression of the equine K+ channel KV11.1 (ether à go-go-related/KCNH2 gene) and the regulatory subunit KCNE2 from equine myocardium. PLoS One. 2015 Sep 16;10(9):e0138320.
IF: 4.411 

35) Poulsen KL, Hotait M, Calloe K, Klaerke DA, Rebeiz A, Nemer G, Tejada MA, Refaat MM. The mutation p.T613A in the pore helix of the KV11.1 potassium channel is associated with long QT syndrome. Pacing Clin Electrophysiol. 2015 Nov;38(11):1304-9
IF: 1.44 

34) Axelsen LN, Calloe K, Braunstein TH, Riemann M, Hofgaard JP, Liang B, Jensen CF, Olsen KB, Baandrup U, Jespersen T, Nielsen LB, Holstein-Rathlou N-H, Nielsen MS. Diet-induced pre-diabetes slows cardiac conductance and promotes arrhythmogenesis. Cardiovasc Diabetol. 2015 Jul 14;14:87.
IF: 4.99 

33) Grubb S, Aistrup G, Koivumäki J, Speerschneider T, Mutsaers N, Olesen SP, Calloe K, Thomsen M. Preservation of cardiac function by prolonged action potentials in mice deficient of KChIP2. Am J Physiol Heart Circ Physiol. 2015 Aug 1;309(3):H481-9. Editorial comment
IF: 3.324 

* 32) Steffensen AB, Refaat M, David J-P, Mujezinovic A, Calloe K, Wojciak J, Nussbaum RL, Scheinman, Schmitt N. High incidence of functional ion-channel abnormalities in a consecutive Long QT cohort with novel missense genetic variants of unknown significance. Sci Rep. 2015 Jun 12;5:10009.
IF: 5.228 

31) Maleckar MM, Lines GT, Koivumäki JT, Cordeiro JM, Calloe K. NS5806 partially restores APD but fails to ameliorate Ca2+ transient dysfunction in a computational model of canine heart failure. Europace. 2014 Nov;16 Suppl 4:iv46-iv55.
IF: 2.94 

30) Hansen DB, Ye ZC, Calloe K, Braunstein TH, Hofgaard JP, Ransom BR, Nielsen MS, MacAulay N. Astrocytic and neuronal large-pore (hemi)channels; Activation, permeability and inhibition. J Biol Chem. 2014 Sep 19;289(38):26058-73. The manuscript was selected as a Journal of Biological Chemistry "Paper of the Week."
IF: 4.258 

29) Grubb S, Speerschneider T, Occhipinti D, Fiset C, Olesen SP, Thomsen MB, Calloe K. Loss of K+ currents in heart failure is accentuated in KChIP2 deficient mice. J Cardiovasc Electrophysiol. 2014 Aug;25(8):896-904.
IF: 3.097 

28) Axelsen LN, Calloe K, Holstein-Rathlou NH, Nielsen MS. Managing the complexity of communication: regulation of gap junctions by post-translational modification. Front Pharmacol. 2013 Oct 22;4:130.
IF: 4.418 

27) Speerschneider T, Grubb S, Metoska A, Olesen SP, Calloe K, Thomsen MB. Development of heart failure is independent of KChIP2 expression. J Physiol. 2013 Dec 1;591(Pt 23):5923-37
IF: 5.037 

26) Dall M, Calloe K, Haupt-Jørgensen M, Larsen J, Schmitt N, Josefsen K, Buschard K. Gliadin fragments and a gliadin 33-mer peptide close the KATP channels and induce insulin secretion – a possible mechanism for the protective gluten-free diet in diabetes. PLOS One. 2013 Jun 13;8(6):e66474.
IF: 4.411 

*25) Calloe K, Goodrow R, Antzelevitch C, Olesen SP, Cordeiro JM, Tissue-specific effects of acetylcholine in the canine heart. Am J Physiol Heart Circ Physiol. 2013 Jul 1;305(1):H66-75.
IF: 3.708 

*24) Olesen MS, Refsgaard L, Holst AG, Larsen AP, Grubb S, Haunsø S, Olesen SP, Schmitt N, Calloe K. A Novel KCND3 gain-of-function mutation associated with early-onset of persistent lone atrial fibrillation. Cardiovasc Res. 2013 Jun 1;98(3):488-95.
IF: 5.465 

23) Gilling M, Rasmussen HB, Calloe K, Sequeira AF, Baretto M, Oliveira G, Almeida J, Lauritsen MB, Ullmann R, Boonen SE, Brondum-Nielsen K, Kalscheuer VM, Tümer Z, Vicente AM, Schmitt N, Tommerup N. Dysfunction of the heteromeric KV7.3/KV7.5 potassium channel is associated with autism spectrum disorders. Front Genet. 2013 Apr 16;4:54.
IF: 3.92 

*22) Calloe K*, Refaat MM*, Grubb S, Wojciak J, Carroll J, Thomsen NM, Nussbaum RL, Scheinman MM, Schmitt N. Characterization and Mechanisms of Action of Novel NaV1.5 Channel Mutations Associated with Brugada Syndrome. *Contributed equally. Circ Arrhythm Electrophysiol. 2013 Feb 1;6(1):177-84.
IF: 5.947 

21) Grubb S, Calloe K, Thomsen MB. Impact of KChIP2 on cardiac electrophysiology and the progression of heart failure. Review. Front. Physio. 2012 Apr: 3:118.
IF: 4.031 

*20) Cordeiro JM*, Calloe K*, Moise NS, Kornreich B, Giannandrea D, Diego JDM, Olesen SP, Antzelevitch C. Physiological consequences of transient outward K+ current activation during heart failure in canine left ventricle. J Mol Cell Cardiol. 2012 Jun;52(6):1291-8. *Contributed equally.
IF: 4.874 

19) Calloe K, Schmitt N, Pfeiffer R, Anderson P, Kanter R, Cordeiro JM, Antzelevitch C. Multiple arrhythmic syndromes in a newborn due to a novel mutation in SCN5A. Can J Physiol Pharmacol. 2011 Oct;89(10):723-36.
IF: 1.91

18) Calloe K, Nof E, Jespersen T, Chlus N, Di Diego JM, Olesen SP, Antzelevitch C, Cordeiro JM. Comparison of the effects of a transient outward potassium channel activator on currents recorded from atrial and ventricular cardiomyocytes. J Cardiovasc Electrophysiol. 2011 Sep;22(9):1057-66.
IF: 3.097 

17) Olesen MF, Jensen NF, Holst AG, Nielsen JB, Tfelt-Hansen J, Haunsø S, Calloe K, Schmitt N, Hastrup J Svendsen. A novel nonsense variant in Nav1.5 co-factor MOG1 eliminates its sodium current increasing effect. Can J Cardiol. 2011 Jul-Aug;27(4):523.e17-23.
IF: 3.112

16) Lundby A, Jespersen T, Schmitt N, Grunnet M, Olesen SP, Cordeiro JM, Calloe K. Effect of the I_to activator NS5806 on cloned Kv4 channels depends on the accessory protein KChIP2. British J of Pharm. 2010; 160(8):2028-44.
IF: 5.259 

15) Nof E, Cordeiro JM, Pérez GJ, Scornik FS, Calloe K, Love B, Burashnikov E, Caceres G, Gunsburg M, Antzelevitch C. (2010) A common single nucleotide polymorphism can exacerbate Long QT type 2 syndrome leading to sudden infant death.  Circ: Cardiovasc Genet. 2010; 3(2):199-206.
IF: 6.728 

14) Calloe K, Soltysinska E, Jespersen T, Lundby A, Antzelevitch C, Olesen SP, Cordeiro JM. Differential effects of the transient outward K(+) current activator NS5806 in the canine left ventricle. J Mol Cell Cardiol. 2010; 48:191-200.
IF: 4.874 

13) Holst AG, Calloe K, Jespersen T, Cedergreen P, Winkel BG, Jensen HK, Leren TP, Haunso S, Svendsen JH, Tfelt-Hansen J. A novel SCN5A mutation in a patient with coexistence of Brugada Syndrome traits and ischaemic heart disease. Case Report Med. 2009:9636-45.
IF: 0.5 

12) Einarsen K, Calloe K. Grunnet M, Olesen SP, Schmitt N. Functional properties of human neuronal Kv11 channels. Pflugers Arch. 2009; 458(4):689-70.
IF: 3.760 

11) Cordeiro JM, Marieb M, Pfeiffer R, Calloe K, Burashnikov E, Antzelevitch C. Accelerated inactivation of the L-type calcium current due to a mutation in CACNB2b underlies the development of a Brugada ECG phenotype J Mol Cell Cardiol. 2009; 46(5):695-703.
IF: 4.874 

10) Calloe K*, Cordeiro JM*, Di Diego JM, Hansen RS, Grunnet M, Olesen SP, Antzelevitch C. A transient outward potassium current activator recapitulates the electrocardiographic manifestations of Brugada syndrome. Cardiovasc Res. 2009; 81(4):686-94. *Contributed equally
IF: 5.465 

9) Calloe K, Ravn LS, Schmitt N, Sui JL, Duno M, Haunso S, Grunnet M, Svendsen JH, Olesen SP. Characterizations of a loss-of-function mutation in the Kir3.4 channel subunit. Biochem. Biophys. Res. Commun. 2007; 364, 889-895.
IF: 2.371 

8) Calloe K, Nielsen MS, Grunnet M, Schmitt N, Jorgensen NK. KCNQ channels are involved in the regulatory volume decrease response in primary neonatal rat cardiomyocytes. Biochim. Biophys. Acta. 2007; 1773(6):764-73.
IF: 5.34 

7) Schmitt N*, Calloe K*, Nielsen NH, Buschmann M, Speckmann EJ, Schulze-Bahr E, Schwarz M. The novel C-terminal KCNQ1 mutation M520R alters protein trafficking. Biochem. Biophys. Res. Commun. 2007; 358, 304-310.*Contributed equally.
IF: 2.371 

6) Elmedyb P, Calloe K, Schmitt N, Hansen RS, Grunnet M, Olesen SP. Modulation of ERG channels by XE991. Basic Clin. Pharmacol. Toxicol. 2007; 100, 316-322.
IF: 3.097 

5) Bentzen BH, Nardi A, Calloe K, Madsen LS, Olesen SP, Grunnet M. The small molecule NS11021 is a potent and specific activator of Ca2+-activated big-conductance K+ channels. Mol. Pharmacol. 2007; 72, 1033-1044.
IF: 3.95

4) Bentzen BH, Schmitt N, Calloe K, Dalby BW, Grunnet M, Olesen SP. The acrylamide (S)-1 differentially affects Kv7 (KCNQ) potassium channels. Neuropharmacology 2006; 51, 1068-1077.
IF: 3.26 

3) Calloe K, Elmedyb P, Olesen SP, Jorgensen NK, Grunnet M. Hypoosmotic cell swelling as a novel mechanism for modulation of cloned HCN2 channels. Biophys. J 2005; 89, 2159-2169.
IF: 3.632 

2) Grunnet M, Behr ER, Calloe K, Hofman-Bang J, Till J, Christiansen M, McKenna WJ, Olesen SP, Schmitt N. Functional assessment of compound mutations in the KCNQ1 and KCNH2 genes associated with long QT syndrome. Heart Rhythm. 2005; 2, 1238-1249.
IF: 4.391 

1) Jensen HS, Callo K, Jespersen T, Jensen BS, Olesen SP. The KCNQ5 potassium channel from mouse: a broadly expressed M-current like potassium channel modulated by zinc, pH, and volume changes. Brain Res. Mol. Brain Res. 2005; 139, 52-62.
IF: 2.83