Kirstine Callø

Associate professor, Ph.D.
kirstinec@sund.ku.dk  

Research Profile:

The main focus of the research in the Cellular Electrophysiology Laboratory is on electrophysiology and ion channels. In particular, we are interested in how mutations or pharmacological manipulations of ion channels affect the cardiac action potential and how the contraction of the heart is coordinated. We have extensive experience with heterologously expressed ion channels (mammalian cell lines and Xenopus laevis oocytes) as well as with recordings of native ionic currents in isolated cardiomyocytes. Further, we have established methods to record action potentials from multicellular preparations of equine hearts (the wedge model) as well as from various types of stem cells. 

Publications

34) Cordeiro JM*, Calloe K*, Aschar-Sobbi R*, Kim K-H, Korogyi A, Occhipinti D, Backx PH,  Panama BK. Physiological roles of the transient outward current Ito in normal and diseased hearts. Accepted in Frontiers in Bioscience. * Contributed equally to this work. 

33) Grubb S, Aistrup G, Koivumäki J, Speerschneider T, Mutsaers N, Olesen SP, Calloe K, Thomsen M. Preservation of Cardiac Function by Prolonged Action Potentials in Mice Deficient of KChIP2. Accepted in AJP-Heart and Circulatory Physiology. 

32) Steffensen AB, Refaat M, David J-P, Mujezinovic A, Calloe K, Wojciak J, Nussbaum RL, Scheinman, Schmitt N. High incidence of functional ion-channel abnormalities in a consecutive Long QT cohort with novel missense genetic variants of unknown significance. Accepted for publication in Scientific Reports. 

31) Maleckar MM, Lines GT, Koivumäki JT, Cordeiro JM, Calloe K. NS5806 partially restores APD but fails to ameliorate Ca2+ transient dysfunction in a computational model of canine heart failure. Europace. 2014 Nov;16 Suppl 4:iv46-iv55. 

30) Hansen DB, Ye ZC, Calloe K, Braunstein TH, Hofgaard JP, Ransom BR, Nielsen MS, MacAulay N Astrocytic and Neuronal Large-pore (Hemi)channels; Activation, Permeability and Inhibition. J Biol Chem. 2014 Sep 19;289(38):26058-73. The manuscript was selected as a Journal of Biological Chemistry "Paper of the Week."  

29) Grubb S, Speerschneider T, Occhipinti D, Fiset C, Olesen SP, Thomsen MB, Calloe K. Loss of K+ Currents in Heart Failure Is Accentuated in KChIP2 Deficient Mice.J Cardiovasc Electrophysiol. 2014 Aug;25(8):896-904. 

28) Axelsen LN, Calloe K, Holstein-Rathlou NH, Nielsen MS. Managing the complexity of communication: regulation of gap junctions by post-translational modification. Front Pharmacol. 2013 Oct 22;4:130. 

27) Speerschneider T, Grubb S, Metoska A, Olesen SP, Calloe K, Thomsen MB. Development of Heart Failure is independent of KChIP2 expression J Physiol. 2013 Dec 1;591(Pt 23):5923-37. 

26) Dall M, Calloe K, Haupt-Jørgensen M, Larsen J, Schmitt N, Josefsen K, Buschard K. Gliadin fragments and a gliadin 33-mer peptide close the KATP channels and induce insulin secretion – a possible mechanism for the protective gluten-free diet in diabetes. PLoS One. 2013 Jun 13;8(6):e66474. 

25) Calloe K, Goodrow R, Antzelevitch C, Olesen SP, Cordeiro JM, Tissue-specific effects of acetylcholine in the canine heart. Am J Physiol Heart Circ Physiol. 2013 Jul 1;305(1):H66-75. 

24) Olesen MS, Refsgaard L,  Holst AG, Larsen AP, Grubb S, Haunsø S, Olesen SP, Schmitt N, Calloe K. A Novel KCND3 Gain-of-Function Mutation Associated with Early-Onset of Persistent Lone Atrial Fibrillation Cardiovasc Res. Cardiovasc Res. 2013 Jun 1;98(3):488-95.

22) Calloe K*, Refaat MM*, Grubb S, Wojciak J, Carroll J,  Thomsen NM, Nussbaum RL, Scheinman MM,  Schmitt N. Characterization and Mechanisms of Action of Novel NaV1.5 Channel Mutations Associated with Brugada Syndrome. *Contributed equally. Circ Arrhythm Electrophysiol. 2013 Feb 1;6(1):177-84. 

21) Grubb S, Calloe K, Thomsen MB. Impact of KChIP2 on cardiac electrophysiology and the progression of 1 heart failure. Review, Front. Physio. 2012 Apr: 3:118. 

20) Cordeiro JM*, Calloe K*, Moise NS, Kornreich B, Giannandrea D, Diego JDM, Olesen SP, Antzelevitch C. Physiological consequences of transient outward K+  current activation during heart failure in canine left ventricle. J Mol Cell Cardiol. 2012 Jun;52(6):1291-8. *Contributed equally.  

19) Calloe K, Schmitt N, Pfeiffer R, Anderson P, Kanter R, Cordeiro JM, Antzelevitch C. Multiple Arrhythmic Syndromes in a Newborn Due to a Novel Mutation in SCN5A. Can J Physiol Pharmacol. 2011 Oct;89(10):723-36. 

18) Calloe K, Nof E, Jespersen T, Chlus N, Di Diego JM, Olesen SP, Antzelevitch C, Cordeiro JM. Comparison of the Effects of a Transient Outward Potassium Channel Activator on Currents Recorded from Atrial and Ventricular Cardiomyocytes. J Cardiovasc Electrophysiol. 2011 Sep;22(9):1057-66. 

17) Olesen MF, Jensen NF, Holst AG, Nielsen JB, Tfelt-Hansen J, Haunsø S, Calloe K, Schmitt N, Hastrup J Svendsen. A novel nonsense variant in Nav1.5 co-factor MOG1 eliminates its sodium current increasing effect. Can J Cardiol. 2011 Jul-Aug;27(4):523.e17-23. 

16) Lundby A, Jespersen T, Schmitt N, Grunnet M, Olesen SP, Cordeiro JM, Calloe K. Effect of the Ito Activator NS5806 on Cloned Kv4 Channels Depends on the Accessory Protein KChIP2. British J of Pharm. 2010; 160(8):2028-44. 

15) Nof E, Cordeiro JM, Pérez GJ, Scornik FS, Calloe K, Love B, Burashnikov E, Caceres G, Gunsburg M, Antzelevitch C. (2010) A common single nucleotide polymorphism can exacerbate Long QT type 2 syndrome leading to sudden infant death.  Circ: Cardiovasc Gent. 2010; 3(2):199-206. 

14) Calloe K, Soltysinska E, Jespersen T, Lundby A, Antzelevitch C, Olesen SP, Cordeiro JM. Differential effects of the transient outward K(+) current activator NS5806 in the canine left ventricle. J Mol Cell Cardiol. 2010; 48:191-200. 

13) Holst AG, Calloe K, Jespersen T, Cedergreen P, Winkel BG, Jensen HK, Leren TP, Haunso S, Svendsen JH, Tfelt-Hansen J. A Novel SCN5A Mutation in a Patient with Coexistence of Brugada Syndrome Traits and Ischaemic Heart Disease. Case Report Med. 2009:9636-45. 

12) Einarsen K, Calloe K. Grunnet M, Olesen SP, Schmitt N. Functional Properties of Human Neuronal Kv11 Channels. Pflugers Arch. 2009; 458(4):689-70. 

11) Cordeiro JM, Marieb M, Pfeiffer R, Calloe K, Burashnikov E, Antzelevitch C. Accelerated Inactivation of the L-type Calcium Due to a Mutation in CACNB2b Underlies the Development of a Brugada ECG phenotype J Mol Cell Cardiol. 2009; 46(5):695-703.

10) Calloe K*, Cordeiro JM*, Di Diego JM, Hansen RS, Grunnet M, Olesen SP, Antzelevitch C. A transient outward potassium current activator recapitulates the electrocardiographic manifestations of Brugada syndrome. Cardiovasc Res. 2009; 81(4):686-94. *Contributed equally Editorial comment: in Cardiovasc. Res. 2009,  81:635-636

9) Calloe K, Ravn LS, Schmitt N, Sui JL, Duno M, Haunso S, Grunnet M, Svendsen JH, Olesen SP. Characterizations of a loss-of-function mutation in the Kir3.4 channel subunit. Biochem. Biophys. Res. Commun. 2007; 364, 889-895.

8) Calloe K, Nielsen MS, Grunnet M, Schmitt N, Jorgensen NK. KCNQ channels are involved in the regulatory volume decrease response in primary neonatal rat cardiomyocytes. Biochim. Biophys. Acta. 2007; 1773(6):764-73.

7) Schmitt N*, Calloe K*, Nielsen NH, Buschmann M, Speckmann EJ, Schulze-Bahr E, Schwarz M. The novel C-terminal KCNQ1 mutation M520R alters protein trafficking. Biochem. Biophys. Res. Commun. 2007; 358, 304-310.*Contributed equally

6) Elmedyb P, Calloe K, Schmitt N, Hansen RS, Grunnet M, Olesen SP. Modulation of ERG Channels by XE991. Basic Clin. Pharmacol. Toxicol. 2007; 100, 316-322.

5) Bentzen BH, Nardi A, Calloe K, Madsen LS, Olesen SP, Grunnet M. The small molecule NS11021 is a potent and specific activator of Ca2+-activated big-conductance K+ channels. Mol. Pharmacol. 2007; 72, 1033-1044.

4) Bentzen BH, Schmitt N, Calloe K, Dalby BW, Grunnet M, Olesen SP. The acrylamide (S)-1 differentially affects Kv7 (KCNQ) potassium channels. Neuropharmacology 2006; 51, 1068-1077.

3) Calloe K, Elmedyb P, Olesen SP, Jorgensen NK, Grunnet M. Hypoosmotic cell swelling as a novel mechanism for modulation of cloned HCN2 channels. Biophys. J 2005; 89, 2159-2169.

2) Grunnet M, Behr ER, Calloe K, Hofman-Bang J, Till J, Christiansen M, McKenna WJ, Olesen SP, Schmitt N. Functional assessment of compound mutations in the KCNQ1 and KCNH2 genes associated with long QT syndrome. Heart Rhythm. 2005; 2, 1238-1249.

1) Jensen HS, Callo K, Jespersen T, Jensen BS, Olesen SP. The KCNQ5 potassium channel from mouse: a broadly expressed M-current like potassium channel modulated by zinc, pH, and volume changes. Brain Res. Mol. Brain Res. 2005; 139, 52-62.